Cell Lines and Cell Cultures
Eurofins Discoverx ChemiScreen NK1 Tackykinin Receptor Stable Cell Line, 2 vials. (HTS080C)
The ChemiScreen NK1 Tachykinin Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. tachykinins) based activation of TACR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses TACR1 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of TACR1, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen D2 Dopamine Receptor Membrane Preparation, 1 vial. (HTS039M)
The ChemiScreen D2 Dopamine Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of DRD2 surface expression. ChemiScreen membrane preps can be used to screen for GPCR activity using GTPγS assays. Increased GPCR activation levels will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx ChemiScreen V2 Vasopressin Receptor Stable Cell Line, 2 vials. (HTS060C)
The ChemiScreen V2 Vasopressin Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. vasopressin) based activation of AVPR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses AVPR2 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of AVPR2, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen FPRL1 N-Formylpeptide Receptor Membrane Preparation, 1 vial. (HTS056M)
The ChemiScreen FPRL1 N-Formylpeptide Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of FPR2 surface expression. ChemiScreen membrane preps can be used to screen for GPCR activity using GTPγS assays. Increased GPCR activation levels will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx ChemiScreen NOP/ORL1 Opioid Receptor Stable Cell Line, 2 vials. (HTS040C)
The ChemiScreen NOP/ORL1 Opioid Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. orphanin FQ) based activation of OPRL1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses OPRL1 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of OPRL1, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiBrite FFA1/GPR40 Free Fatty Acid Receptor Stable Cell Line, 2 vials. (HTS038L)
The ChemiBrite FFA1/GPR40 Free Fatty Acid Receptor cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. free fatty acids) based activation of FFAR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses FFAR1, a naturally Gq coupled GPCR. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of FFAR1, the resulting calcium activity can be assayed in either detection mode. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx Ready-to-Assay M1 Acetylcholine (Muscarinic) Receptor Frozen Cells, 2 Vials of Cells. (HTS044RTA)
The Ready-to-Assay M1 Acetylcholine (Muscarinic) Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. acetylcholine) based activation of CHRM1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CHRM1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CHRM1, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx Ready-to-Assay PAF Platelet Activating Factor Receptor Frozen Cells, 2 Vials of Cells. (HTS076RTA)
The Ready-to-Assay PAF Platelet Activating Factor Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. PAF) based activation of PTAFR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTAFR alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of PTAFR, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen NOP/ORL1 Opioid Receptor Membrane Preparation, 1 vial. (HTS040M)
The ChemiScreen NOP/ORL1 Opioid Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of OPRL1 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx Ready-to-Assay NTR1 Neurotensin Receptor Frozen Cells, 2 Vials of Cells. (HTS034RTA)
The Ready-to-Assay NTR1 Neurotensin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. neurotensin) based activation of NTSR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses NTSR1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of NTSR1, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen PTH1 Parathyroid Hormone Receptor Stable Cell Line, 2 vials. (HTS030C)
The ChemiScreen PTH1 Parathyroid Hormone Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. parathyroid hormone) based activation of PTH1R GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTH1R alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of PTH1R, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx ChemiScreen GPR14 Urotensin II Receptor Membrane Preparation, 1 vial. (HTS033M)
The ChemiScreen GPR14 Urotensin II Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of UTS2R surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx Ready-to-Assay β1 Adrenergic Receptor Frozen Cells, 2 Vials of Cells. (HTS104RTA)
The Ready-to-Assay β1 Adrenergic Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. epinephrine and norepinephrine) based activation of ADRB1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses ADRB1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of ADRB1, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
Eurofins Discoverx ChemiScreen α2A Adrenergic Receptor Membrane Preparation, 1 vial. (HTS096M)
The ChemiScreen α2A Adrenergic Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of ADRA2A surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx ChemiScreen D3 Dopamine Receptor Membrane Preparation, 1 vial. (HTS103M)
The ChemiScreen D3 Dopamine Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of DRD3 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.